Currently parameters obtained through the analysis of spermatozoids (seminogram) do not give the complete picture regarding the fertility potential of semen or the capacity to create a healthy embryo for a normal pregnancy.A complete study of the male factor and its contribution to the fertility problem in couples requires determining some other parameters like:
FISH in spermatozoids
Via the FISH technique, explained previously, we check if any chromosomal changes have taken place (aneuploidy) in spermatozoids. An increase in the number of altered spermatozoids might be responsible for failed implantations and miscarriages.
Sieving of microdeletion of the Y chromosome:
We can identify through the PCR technique possible deletion of the Y chromosome in different, specific regions. It has been proven that absence in these regions is responsible for certain alterations in gametogenesis.
Determination of the Spermatic DNA fragmentation index (TUNEL):
It has been proven that infertile males have a great fraction of broken DNA spermatozoids and that its impact can translate into negative assisted reproduction results.
The technique that we use to determine spermatic DNA fragmentation is called TUNEL or Terminal dUTP Nick-End Labeling that consists of measuring broken DNA chains incorporating fluorecently marked molecules.